| Объем | 100 мкг |
| Синонимы | Protein N-terminal glutamine amidohydrolase (EC 3.5.1.122) (Protein NH2-terminal glutamine deamidase) (N-terminal Gln amidase) (Nt(Q)-amidase) (WDYHV motif-containing protein 1), WDYHV1, C8orf32 NTAQ1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q96HA8 |
| Иммуноген | Recombinant Human Protein N-terminal glutamine amidohydrolase protein (1-205AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, Recommended dilution: WB:1:500-1:2000 |
| Примечание | Mediates the side-chain deamidation of N-terminal glutamine residues to glutamate, an important step in N-end rule pathway of protein degradation. Conversion of the resulting N-terminal glutamine to glutamate renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. Does not act on substrates with internal or C-terminal glutamine and does not act on non-glutamine residues in any position. Does not deaminate acetylated N-terminal glutamine. With the exception of proline, all tested second-position residues on substrate peptides do not greatly influence the activity. In contrast, a proline at position 2, virtually abolishes deamidation of N-terminal glutamine (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: WDYHV1 antibody at 6µg/ml
Lane 1: Mouse heart tissue
Lane 2: MCF-7 whole cell lysate
Lane 3: U251 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 24, 17 kDa
Observed band size: 24 kDa
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Наименование: WDYHV1 Antibody.