Объем | 50 мкг |
Синонимы | WAP four-disulfide core domain protein 2 (Epididymal secretory protein E4) (Major epididymis-specific protein E4) (Putative protease inhibitor WAP5), WFDC2, HE4 WAP5 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q14508 |
Иммуноген | Recombinant Human WAP four-disulfide core domain protein 2 protein (31-124AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | WAP four-disulfide core domain protein 2 protein |
Области исследований | Tags & Cell Markers |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: HepG2 cell, Mouse heart
All lanes: WFDC2 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 13, 9, 12 kDa
Observed band size: 13 kDa
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA09794A0Rb at dilution of 1:100
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Immunofluorescent analysis of 293T cells using CSB-PA09794A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunofluorescent analysis of MCF-7 cells using CSB-PA09794A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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IHC image of CSB-PA09794A0Rb diluted at 1:200 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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