Объем | 100 мкг |
Синонимы | Vesicle transport protein USE1 (Putative MAPK-activating protein PM26) (USE1-like protein) (p31), USE1, USE1L |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q9NZ43 |
Иммуноген | Recombinant Human Vesicle transport protein USE1 protein (1-146AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:500, IP:1:200-1:2000 |
Примечание | SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Vesicle transport protein USE1 |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Jurkat whole cell lysate, Mouse heart tissue, Mouse brain tissue, Mouse kidney tissue, Mouse stomach tissue, Mouse skeletal muscle tissue
All lanes: USE1 antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 30, 17, 16 kDa
Observed band size: 36 kDa
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Immunofluorescent analysis of U251 cells using CSB-PA889157LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating USE1 in PC-3 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA889157LA01HU in PC-3 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA889157LA01HU (6µg) + PC-3 whole cell lysate (500µg)
Lane 3: PC-3 whole cell lysate (10µg)
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