| Объем | 100 мкг |
| Синонимы | 14-3-3 protein gamma (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein gamma, N-terminally processed], YWHAG |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P61981 |
| Иммуноген | Recombinant Human 14-3-3 protein gamma protein (113-194AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | 14-3-3 protein gamma |
| Области исследований | Neuroscience, Cancer, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA026288LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA026288LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: Mouse brain tissue, A431 whole cell lysate, Human placenta tissue
All lanes: YWHAG antibody at 3.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
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Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate, U251 whole cell lysate, K562 whole cell lysate, Rat brain tissue
All lanes: YWHAG antibody at 3.3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
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Immunoprecipitating YWHAG in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA026288LA01HU in NIH/3T3 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA026288LA01HU (5µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (20µg)
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Наименование: YWHAG Antibody.