| Объем | 100 мкл |
| Синонимы | Vitronectin (VN) (S-protein) (Serum-spreading factor) (V75) [Cleaved into: Vitronectin V65 subunit, Vitronectin V10 subunit, Somatomedin-B], VTN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P04004 |
| Иммуноген | Recombinant Human Vitronectin protein (364-478AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Области исследований | Cardiovascular, Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Vitronectin antibody at 12µg/ml + HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
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Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA025944ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA025944ESR1HU at dilution of 1:100
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Immunoprecipitating VTN in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA025944ESR1HU in NIH/3T3 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA025944ESR1HU (8µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (10µg)
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Наименование: VTN Antibody.