| Объем | 50 мкг |
| Синонимы | 14-3-3 protein beta/alpha (Protein 1054) (Protein kinase C inhibitor protein 1) (KCIP-1) [Cleaved into: 14-3-3 protein beta/alpha, N-terminally processed], YWHAB |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P31946 |
| Иммуноген | Recombinant Human 14-3-3 protein beta/alpha protein (1-246AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat, Zebrafish |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:50-1:500 |
| Примечание | Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation amoung many other functions. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | 14-3-3 protein beta/alpha protein |
| Области исследований | Neuroscience, Cell biology, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: 14-3-3 protein beta/alpha antibody at 2µg/ml
Lane 1: 293T whole cell lysate
Lane 2: EC109 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 29, 28 kDa
Observed band size: 29 kDa
 |
Western blot
All lanes: YWHAB antibody at 6µg/ml
Lane 1: Hela whole cell lysate
Lane 2: Raji whole cell lysate
Lane 3: Mouse liver tissue
Lane 4: Mouse brain tissue
Lane 5: Rat liver tissue
Lane 6: Rat lung tissue
Lane 7: Mouse lung tissue
Lane 8: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 29, 28 kDa
Observed band size: 28, 39, 64 kDa
 |
Western Blot
Positive WB detected in: Jurkat whole cell lysate, HepG2 whole cell lysate
All lanes: YWHAB antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29, 28 kDa
Observed band size: 29 kDa
 |
IHC image of CSB-PA01544A0Rb diluted at 1:600 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA01544A0Rb diluted at 1:600 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescent analysis of HepG2 cells using CSB-PA01544A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, 293T whole cell lysate, Jurkat whole cell lysate, HT29 whole cell lysate, Rat brain tissue
All lanes: YWHAB antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
 |
IHC image of CSB-PA01544A0Rb diluted at 1:400 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: YWHAB Antibody.