AKT1 (Ab-473) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | AKT, C-AKT, PKB, PKB-alpha, RAC | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P31749 | | Иммуноген | Peptide sequence around aa. 471~475 (Q-F-S-Y-S) derived from Human Akt. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC,WB:1:500-1:1000, IHC:1:50-1:200 | | Примечание | General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. <br/><br/>Baudhuin LM, et al. (2004) FASEB J Feb, 18(2): 341-3.<br/>Min YH, et al. (2004) Cancer Res, 64(15): 5225-31.<br/>Feng J, et al. (2004) J Biol Chem, 279(34): 35510-7.<br/>Ayala G, et al. (2004) Clin Cancer Res, 10(19): 6572-8.<br/>Lungu AO, et al. (2004) J Biol Chem, 279(47): 48794-800. <br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Neuroscience,Cancer,Metabolism,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293 and 3T3 cells using Akt(Ab-473) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Ab-473) Antibody(left) or the same antibody preincubated with blocking peptide(right).
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Наименование: AKT1 (Ab-473) Antibody.