ATF2 (Ab-62 or 44) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CREB2, CREBP1, | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P15336 | | Иммуноген | Peptide sequence around aa.serine 60~64 or 42~46 (N-D-S-V-I) derived from Human ATF2. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:1000, IHC:1:50-1:200, IF:1:100-1:200 | | Примечание | Transcriptional activator, probably constitutive, which binds to the cAMP-responsive element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Interaction with JUN redirects JUN to bind to CRES preferentially over the 12-O-tetradecanoylphorbol-13-acetate response elements (TRES) as part of an ATF2-c-Jun complex.<br/><br/>Sevilla A, et al. (2004) J Biol Chem. 279(26):27458-27465.<br/>Sakurai A, et al. (1991) Biochem Biophys Res Commun. 181(2): 629-635.<br/>Abdel-Hafiz H A, et al. (1992) Mol Endocrinol. 6: 2079-2089.<br/>Gupta S, et al. (1995) Science. 267: 389-393.<br/>Van Dam H, et al. (1995) EMBO J. 14(8): 1798-1811.<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific peptide. | | Области исследований | Epigenetics and Nuclear Signaling,Immunology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from 293 and 3T3 cells using ATF2(Ab-62 or 44) Antibody.
 | Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Ab-62 or 44) Antibody(left) or the same antibody preincubated with blocking peptide(right).
 | Immunofluorescence staining of methanol-fixed Hela cells using ATF2(Ab-62 or 44) Antibody.
 | | | |
Информация для заказа |
Наименование: ATF2 (Ab-62 or 44) Antibody.