| Объем | 50 мкг |
| Синонимы | ATP synthase subunit alpha, mitochondrial, ATP5A1, ATP5A ATP5AL2 ATPM |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P25705 |
| Иммуноген | Recombinant Human ATP synthase subunit alpha, mitochondrial protein (44-553AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, Recommended dilution: WB:1:500-1:2000 |
| Примечание | Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Tags & Cell Markers, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: ATP5A1 antibody at 2µg/ml
Lane 1: Hela whole cell lysate
Lane 2: COLO205 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60, 55, 58 kDa
Observed band size: 60 kDa
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Наименование: ATP5A1 Antibody.