| Объем | 100 мкг |
| Синонимы | Bone morphogenetic protein 2 (BMP-2) (Bone morphogenetic protein 2A) (BMP-2A), BMP2, BMP2A |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P12643 |
| Иммуноген | Recombinant Human Bone morphogenetic protein 2 protein (283-396AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Induces cartilage and bone formation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Cardiovascular, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA09419A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA09419A0Rb at dilution of 1:100
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Western blot
All lanes: BMP2 antibody at 7.5µg/ml + A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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Immunofluorescent analysis of Hela cells using CSB-PA09419A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Western Blot
Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate
All lanes: BMP2 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
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IHC image of CSB-PA09419A0Rb diluted at 1:750 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: BMP2 Antibody.