| Объем | 100 мкг |
| Синонимы | Carbonic anhydrase 1 (EC 4.2.1.1) (Carbonate dehydratase I) (Carbonic anhydrase B) (CAB) (Carbonic anhydrase I) (CA-I), CA1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P00915 |
| Иммуноген | Recombinant Human Carbonic anhydrase 1 protein (2-261AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cardiovascular, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Carbonic anhydrase 1 antibody at 2µg/ml
Lane 1: K562 whole cell lysate
Lane 2: jurkat whole cell lysate
Lane 3: Mouse liver tissue
Secondary
Goat polyclonal to rabbit lgG at 1/15000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
 |
Western Blot
Positive WB detected in: Rat lung tissue, Rat kidney tissue, Mouse lung tissue, Mouse kidney tissue
All lanes: Ca1 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
 |
Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA004364HA01HU at dilution of 1:100
 |
Immunofluorescent analysis of A549 cells using CSB-PA004364HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
 |
Western Blot
Positive WB detected in: K562 whole cell lysate, Mouse spleen tissue, Mouse kidney tissue
All lanes: Ca1 antibody at 6.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa
 |
Immunoprecipitating Ca1 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA004364HA01HU in K562 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA004364HA01HU (8µg) + K562 whole cell lysate (500µg)
Lane 3: K562 whole cell lysate (20µg)
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Наименование: CA1 Antibody.