| Объем | 50 мкг |
| Синонимы | Calreticulin (CRP55) (Calregulin) (Endoplasmic reticulum resident protein 60) (ERp60) (HACBP) (grp60), CALR, CRTC |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P27797 |
| Иммуноген | Recombinant Human Calreticulin protein (18-417AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Tags & Cell Markers |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CALR antibody at 2µg/ml
Lane 1: NIH/3T3 whole cell lysate
Lane 2: Colo320 whole cell lysate
Lane 3: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa
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Immunohistochemistry of paraffin-embedded human thyroid tissue using CSB-PA07867A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA07867A0Rb at dilution of 1:100
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Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, PC-3 whole cell lysate, Rat kidney tissue, Mouse kidney tissue
All lanes: CALR antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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Immunofluorescent analysis of Hela cells using CSB-PA07867A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: CALR Antibody.