Объем | 100 мкг |
Синонимы | Calcium signal-modulating cyclophilin ligand (CAML), CAMLG, CAML |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P49069 |
Иммуноген | Recombinant Human Calcium signal-modulating cyclophilin ligand protein (1-189AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, IP, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:500, IP:1:200-1:2000 |
Примечание | Likely involved in the mobilization of calcium as a result of the TCR/CD3 complex interaction. Binds to cyclophilin B. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Calcium signal-modulating cyclophilin ligand |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Jurkat whole cell lysate, HEK293 whole cell lysate, U87 whole cell lysate, SH-SY5Y whole cell lysate
All lanes: CAMLG antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 33 kDa
Observed band size: 33 kDa
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Immunofluorescent analysis of HepG2 cells using CSB-PA004475LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating CAMLG in HEK293 whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA004475LA01HU in HEK293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA004475LA01HU (8µg) + HEK293 whole cell lysate (500µg)
Lane 3: HEK293 whole cell lysate (10µg)
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