| Объем | 100 мкг |
| Синонимы | Calpain-1 catalytic subunit (EC 3.4.22.52) (Calcium-activated neutral proteinase 1) (CANP 1) (Calpain mu-type) (Calpain-1 large subunit) (Cell proliferation-inducing gene 30 protein) (Micromolar-calpain) (muCANP), CAPN1, CANPL1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P07384 |
| Иммуноген | Recombinant Human Calpain-1 catalytic subunit protein (1-714AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Calpain-1 catalytic subunit |
| Области исследований | Cell biology, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CAPN1 antibody at 6µg/ml
Lane 1: A375 whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: PC-3 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: Hela whole cell lysate
Lane 6: A2780 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 82 kDa
Observed band size: 82 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA004490LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA004490LA01HU at dilution of 1:100
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Immunofluorescence staining of MCF-7 cells with CSB-PA004490LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: CAPN1 Antibody.