Объем | 50 мкг |
Синонимы | Calpain small subunit 2 (CSS2) (Calcium-dependent protease small subunit 2), CAPNS2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q96L46 |
Иммуноген | Recombinant Human CAPNS2 protein (1-248AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:2000, IF:1:50-1:200 |
Примечание | Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. This small subunit may act as a tissue-specific chaperone of the large subunit, possibly by helping it fold into its correct conformation for activity. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cell biology, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CAPNS2 antibody at 8µg/ml + Mouse heart tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
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Immunofluorescence staining of A549 cells with CSB-PA822248LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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