Объем | 50 мкг |
Синонимы | G2/mitotic-specific cyclin-B2, CCNB2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | O95067 |
Иммуноген | Recombinant Human G2/mitotic-specific cyclin-B2 protein (7-163AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
Примечание | Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | G2/mitotic-specific cyclin-B2 |
Области исследований | Epigenetics and Nuclear Signaling, Cell biology |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: A549 whole cell lysate
All lanes: CCNB2 antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46 kDa
Observed band size: 46 kDa
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Immunofluorescence staining of Hela cells with CSB-PA004808LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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