Объем | 100 мкл |
Синонимы | T-complex protein 1 subunit epsilon (TCP-1-epsilon) (CCT-epsilon), CCT5, CCTE KIAA0098 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P48643 |
Иммуноген | Recombinant Human T-complex protein 1 subunit epsilon protein (1-270AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
Примечание | Molecular chaperone, assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | Antigen Affinity Purified |
Абревеатура | T-complex protein 1 subunit epsilon |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CCT5 antibody at 1.78µg/ml
Lane 1: Hela whole cell lysate
Lane 2: Colo320 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 60, 50 kDa
Observed band size: 60 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA004862ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA004862ESR1HU at dilution of 1:100
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Immunoprecipitating CCT5 in Hela whole cell lysate
Lane 1: Rabbit control IgG (1µg) instead of CSB-PA004862ESR1HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA004862ESR1HU (8µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (10µg)
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