| Объем | 50 мкл |
| Синонимы | Calcium-binding and coiled-coil domain-containing protein 2 (Antigen nuclear dot 52 kDa protein) (Nuclear domain 10 protein NDP52) (Nuclear domain 10 protein 52) (Nuclear dot protein 52), CALCOCO2, NDP52 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q13137 |
| Иммуноген | Recombinant Human Calcium-binding and coiled-coil domain-containing protein 2 protein (137-446AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting galectin-8-associated bacteria for autophagy (PubMed:22246324). Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation (PubMed:23022382, PubMed:25771791). Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C (PubMed:23022382, PubMed:25771791). May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion (PubMed:17635994). |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Calcium-binding and coiled-coil domain-containing protein 2 |
| Области исследований | Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CALCOCO2 antibody at 3.66µg/ml
Lane 1: Hela whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: 293T whole cell lysate
Lane 4: Raji whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 53, 48, 55, 56, 44 kDa
Observed band size: 56 kDa
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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA614394ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA614394ESR2HU at dilution of 1:100
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Immunoprecipitating CALCOCO2 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA614394ESR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was uses the Secondary antibody (1/50000)
Lane 2: CSB-PA614394ESR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: CALCOCO2 Antibody.