| Объем | 100 мкг |
| Синонимы | Calpastatin (Calpain inhibitor) (Sperm BS-17 component), CAST |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P20810 |
| Иммуноген | Recombinant Human Calpastatin protein (1-667AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:10-1:500, IF:1:50-1:200 |
| Примечание | Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience, Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: Calpastatin antibody at 10µg/ml
Lane 1: K562 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: A549 whole cell lysate
Lane 4: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 77, 76, 64, 72, 82, 85, 83, 75, 81 kDa
Observed band size: 77 kDa
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Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA004561YA01HU at dilution of 1:10
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Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA004561YA01HU at dilution of 1:10
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Western Blot
Positive WB detected in: HL60 whole cell lysate
All lanes: CAST antibody at 2.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 77, 76, 64, 72, 82, 85, 83, 75, 81 kDa
Observed band size: 77 kDa
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IHC image of CSB-PA004561YA01HU diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA004561YA01HU diluted at 1:500 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of A549 cells with CSB-PA004561YA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: CAST Antibody.