Объем | 50 мкг |
Синонимы | T-complex protein 1 subunit epsilon, TCP-1-epsilon, CCT-epsilon, CCT5, CCTE, KIAA0098 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | P48643 |
Иммуноген | Recombinant Human T-complex protein 1 subunit epsilon protein (1-270AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
Примечание | Molecular chaperone, assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | T-complex protein 1 subunit epsilon |
Области исследований | Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate
All lanes: CCT5 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60, 50 kDa
Observed band size: 60 kDa
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Immunofluorescence staining of A549 cells with CSB-PA004862EA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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