| Объем | 100 мкл |
| Синонимы | T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, CD4 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | P01730 |
| Иммуноген | A synthesized peptide |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:500 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Immunology |
| Аббревиатура | T-cell surface glycoprotein CD4 |
| Примечание | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in:Jurkat whole cell lysate
All lanes:CD4 antibody at 0.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
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IHC image of CSB-RA004935A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-RA004935A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: CD4 Antibody.