Объем | 50 мкг |
Синонимы | CD99 antigen-like protein 2 (MIC2-like protein 1) (CD antigen CD99), CD99L2, MIC2L1 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q8TCZ2 |
Иммуноген | Recombinant Human CD99 antigen-like protein 2 protein (26-145AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 |
Примечание | Plays a role in a late step of leukocyte extravasation helping cells to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1 (By similarity). Homophilic adhesion molecule, but these interactions may not be required for cell aggregation (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | CD99 antigen-like protein 2 |
Области исследований | Immunology, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA840569LA01HU diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system.
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Immunofluorescence staining of A549 cells with CSB-PA840569LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate
All lanes: CD99L2 antibody at 4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 28, 23, 21, 16, 29 kDa
Observed band size: 28 kDa
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