| Объем | 50 мкл |
| Code NO# | 9H2D3 |
| Синонимы | Cadherin-1 (CAM 120/80) (Epithelial cadherin) (E-cadherin) (Uvomorulin) (CD antigen CD324) [Cleaved into: E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3], CDH1, CDHE UVO |
| Тип антител | Monoclonal Antibody |
| Видовая специфичность | Human |
| Uniport ID | P12830 |
| Иммуноген | Recombinant Human Cadherin-1 protein (155-707AA) |
| Источник | Mouse |
| Видовая специфичность 1 | Human, Mouse |
| Применение | ELISA, WB, IF, FC, Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200 |
| Примечание | Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. |
| Изотип | IgG1 |
| Метод очистки | >95%, Protein G purified |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Области исследований | Cancer, Developmental biology, Signal transduction |
| Станица на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Caco205 whole cell lysate
All lanes: CDH1 antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 98, 91 kDa
Observed band size: 110, 120 kDa
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Immunofluorescence staining of Hela cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescence staining of MCF-7 cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Immunofluorescence staining of SW620 cells with CSB-MA005034A0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).
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Overlay histogram showing MCF-7 cells stained with CSB-MA005034A0m (red line) at 1:100. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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Наименование: CDH1 Monoclonal Antibody.