CDKN2A AntibodyСпецификация| Объем | 100 мкл | | Синонимы | CDK4I, CMM2, MLM, MTS1, TP16 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q8N726 | | Иммуноген | Synthesized peptide derived from human p14 ARF antibody. | | Источник | Rabbit | | Видовая специфичность | Human | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 6 may be involved in regulation of autophagy and caspase-independent cell death, the short-lived mitochondrial isoform isstabilized by C1QBP. UniProtKB Q64364<br/><br/>B篓顩噒rice Eymin, Mol. Cell. Biol., Jun 2006, 26: 4339 - 4350. <br/>Guillaume Normand, J. Biol. Chem., Feb 2005, 280: 7118 - 7130. <br/>Abel S篓顣攃hez-Aguilera, Blood, Feb 2002, 99: 1411 - 1418. <br/>Manel Esteller, Cancer Res., Apr 2001, 61: 2816 - 2821. <br/><br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemical analysis of paraffin-embedded human placenta tissue using p14 ARF antibody.
 | Immunofluorescence analysis of HeLa cells, using p14 ARF antibody.
 | Western blot analysis of extracts from HeLa cells, using p14 ARF antibody.
 | Western blot analysis of extracts from 293 cells (Lane 2), using p14 ARF antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: CDKN2A Antibody.