CHFR AntibodyСпецификация| Объем | 100 мкл | | Синонимы | checkpoint with forkhead and ring finger domains, Checkpoint with forkhead and RING finger domains protein, CHFR, E3 ubiquitin-protein ligase CHFR, FLJ10796 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q96EP1 | | Иммуноген | Synthesized peptide derived from Internal of human CHFR. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB,WB:1:500-1:3000 | | Примечание | E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.<br/><br/>Scolnick D.M., Nature 406:430-435(2000).<br/>Ota T., Nat. Genet. 36:40-45(2004).<br/>Scherer S.E., Nature 440:346-351(2006)<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling,Cancer,Cell biology | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from Jurkat cells, using CHFR antibody.
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Наименование: CHFR Antibody.