| Объем | 100 мкг |
| Синонимы | Choline kinase alpha (CK) (EC 2.7.1.32) (CHETK-alpha) (Ethanolamine kinase) (EK) (EC 2.7.1.82), CHKA, CHK CKI |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P35790 |
| Иммуноген | Recombinant Human Choline kinase alpha protein (79-173AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Has a key role in phospholipid biosynthesis and may contribute to tumor cell growth. Catalyzes the first step in phosphatidylcholine biosynthesis. Contributes to phosphatidylethanolamine biosynthesis. Phosphorylates choline and ethanolamine. Has higher activity with choline. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | Choline kinase alpha |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: CHKA antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53, 51 kDa
Observed band size: 53 kDa
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA005353LA01HU at dilution of 1:100
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Immunofluorescence staining of HepG2 cells with CSB-PA005353LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: CHKA Antibody.