| Объем | 100 мкл |
| Синонимы | Calponin-1, Basic calponin, Calponin H1, smooth muscle, CNN1 |
| Тип антител | Recombinant Antibody |
| Species | Human |
| UniProt ID | P51911 |
| Иммуноген | A synthesized peptide derived from human CNN1 |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:5000, IHC:1:50-1:200 |
| Клональность | Monoclonal |
| Изотип | Rabbit IgG |
| Коньюгат | Non-conjugated |
| Буффер | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Affinity-chromatography |
| Области исследований | Signal Transduction |
| Аббревиатура | Calponin-1 |
| Примечание | Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity). |
| Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: 293 whole cell lysate, Mouse lung tissue
All lanes: CNN1 antibody at 1.05µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34, 32 KDa
Observed band size: 34 KDa
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IHC image of CSB-RA005655A0HU diluted at 1:105 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: CNN1 Antibody.