| Объем | 50 мкг |
| Синонимы | Collagen alpha-1(IV) chain [Cleaved into: Arresten], COL4A1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P02462 |
| Иммуноген | Recombinant Human Collagen alpha-1(IV) chain protein (30-167AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA11469A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA11469A0Rb at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human glioma using CSB-PA11469A0Rb at dilution of 1:100
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Immunofluorescence staining of U251 cells with CSB-PA11469A0Rb at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: COL4A1 Antibody.