| Объем | 50 мкл |
| Синонимы | Histone H2A type 1 (H2A.1) (Histone H2A/ptl), HIST1H2AG, HIST1H2AI, HIST1H2AK, HIST1H2AL, HIST1H2AM, H2AFP, H2AFC, H2AFD, H2AFI, H2AFN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P0C0S8 |
| Иммуноген | Peptide sequence around site of crotonyl-Lys(125) derived from Human Histone H2A |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IF, ChIP, Recommended dilution: IF:1:50-1:200 |
| Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Histone H2A type 1 |
| Области исследований | Epigenetics and Nuclear Signaling |
| Ссылка на страницу на сайте производителя | ссылка |
Immunofluorescent analysis of Hela cells (treated with 30mM crotonylate for 4h) tissue using CSB-PA010389PA125crHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
 |
Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium crotonylate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H2AG (CSB-PA010389PA125crHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the ?-Globin promoter.
 |
| | |
Наименование: Crotonyl-HIST1H2AG (K125) Antibody.