| Объем | 50 мкл |
| Синонимы | Casein kinase I isoform gamma-2 (CKI-gamma 2) (EC 2.7.11.1), CSNK1G2, CK1G2 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P78368 |
| Иммуноген | Recombinant Human Casein kinase I isoform gamma-2 protein (316-415AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Casein kinase I isoform gamma-2 |
| Области исследований | Cancer, Metabolism, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CSNK1G2 antibody at 4.21µg/ml
Lane 1: Mouse gonadal tissue
Lane 2: Hela whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA006070ESR2HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA006070ESR2HU at dilution of 1:100
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Immunoprecipitating CSNK1G2 in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA006070ESR2HU in Hela whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used the Secondary antibody (1/50000)
Lane 2: CSB-PA006070ESR2HU (4µg) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
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Наименование: CSNK1G2 Antibody.