Объем | 100 мкг |
Синонимы | Cytoplasmic polyadenylation element-binding protein 4 (CPE-BP4) (CPE-binding protein 4) (hCPEB-4), CPEB4, KIAA1673 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q17RY0 |
Иммуноген | Recombinant Human Cytoplasmic polyadenylation element-binding protein 4 protein (244-515AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200 |
Примечание | cytoplasm, dendrite, endoplasmic reticulum, messenger ribonucleoprotein complex, neuron projection, nucleus, postsynaptic density, synapse, mRNA 3'-UTR binding, poly(A) RNA binding |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Epigenetics and Nuclear Signaling |
Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: CPEB4 antibody at 2µg/ml
Lane 1: Hela whole cell lysate
Lane 2: 293T whole cell lysate
Lane 3: U87 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 81, 79, 37 kDa
Observed band size: 81 kDa
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Immunofluorescence staining of Hela cells with CSB-PA618177LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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