DAZAP2 AntibodyСпецификация| Объем | 100 мкг | | Синонимы | DAZ-associated protein 2 (Deleted in azoospermia-associated protein 2), DAZAP2, KIAA0058 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q15038 | | Иммуноген | Recombinant Human DAZ-associated protein 2 protein (1-168AA) | | Источник | Rabbit | | Видовая специфичность | Human, Rat | | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200 | | Примечание | nuclear speck, identical protein binding, WW domain binding | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | >95%, Protein G purified | | Абревеатура | DAZ-associated protein 2 | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: Hela whole cell lysate, A375 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: DAZAP2 antibody at 3.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 18, 17, 16, 14, 10, 23 kDa
Observed band size: 18 kDa
 | IHC image of CSB-PA622986LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of MCF-7 cells with CSB-PA622986LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: DAZAP2 Antibody.