Объем | 50 мкг |
Синонимы | Disks large homolog 2 (Channel-associated protein of synapse-110) (Chapsyn-110) (Postsynaptic density protein PSD-93), DLG2 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q15700 |
Иммуноген | Recombinant Human Disks large homolog 2 protein (310-420AA) |
Источник | Rabbit |
Видовая специфичность | Human, Rat |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:200-1:500 |
Примечание | Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses (By similarity). |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Disks large homolog 2 |
Области исследований | Neuroscience |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat heart tissue
All lanes: DLG2 antibody at 2.8µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 98, 110, 84, 101, 39 kDa
Observed band size: 98 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA621882LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA621882LA01HU at dilution of 1:100
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Immunofluorescence staining of HepG2 cells with CSB-PA621882LA01HU at 1:233, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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