| Объем | 50 мкг |
| Синонимы | Protein delta homolog 1 (DLK-1) (pG2) [Cleaved into: Fetal antigen 1 (FA1)], DLK1, DLK |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P80370 |
| Иммуноген | Recombinant Human Protein delta homolog 1 protein (24-303AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:200-1:1000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | May have a role in neuroendocrine differentiation. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Neuroscience, Signal transduction, Stem cells |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: protein delta homolog 1 antibody at 2µg/ml + U251 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 42, 34 kDa
Observed band size: 42 kDa
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA006945LA01HU at dilution of 1:100
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Immunofluorescent analysis of A549 cells using CSB-PA006945LA01HU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG
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Western Blot
Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, SH-SY5Y whole cell lysate, Rat brain tissue
All lanes: DLK1 antibody at 4.6µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42, 34 kDa
Observed band size: 42 kDa
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IHC image of CSB-PA006945LA01HU diluted at 1:300 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of HepG2 cells with CSB-PA006945LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: DLK1 Antibody.