| Объем | 50 мкл |
| Синонимы | Protein crumbs homolog 1, CRB1 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P82279 |
| Иммуноген | Recombinant Human Protein crumbs homolog 1 protein (20-200AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, IHC, IF, Recommended dilution: IHC:1:20-1:200, IF:1:10-1:100 |
| Примечание | Plays a role in photoreceptor morphogenesis in the retina. May maintain cell polarization and adhesion. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | Protein crumbs homolog 1 |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
IHC image of CSB-PA005941DSR1HU diluted at 1:66 and staining in paraffin-embedded human eye tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Immunofluorescence staining of Hela cells with CSB-PA005941DSR1HU at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: CRB1 Antibody.