CRYBA1 AntibodyСпецификацияОбъем | 50 мкг | Синонимы | Beta-crystallin A3 [Cleaved into: Beta-crystallin A3, isoform A1, Delta4 form, Beta-crystallin A3, isoform A1, Delta7 form, Beta-crystallin A3, isoform A1, Delta8 form], CRYBA1, CRYB1 | Клональность | Polyclonal Antibody | Организм | Human | uniprot | P05813 | Иммуноген | Recombinant Human Beta-crystallin A3 protein (1-215AA) | Источник | Rabbit | Видовая специфичность | Human, Mouse | Применение | ELISA, WB, IF, Recommended dilution: WB:1:1000-1:5000, IF:1:50-1:200 | Примечание | Crystallins are the dominant structural components of the vertebrate eye lens. | Клональность1 | Polyclonal | Изотип | IgG | Коньюгат | Non-conjugated | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 | Форма | Liquid | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | Метод очистки | >95%, Protein G purified | Области исследований | Neuroscience | Ссылка на страницу на сайте производителя | ссылка | Western blot
All lanes: CRYBA1 antibody at 6µg/ml
Lane 1: Mouse heart tissue
Lane 2: Human placenta tissue
Lane 3: U251 whole cell lysate
Lane 4: U937 whole cell lysate
Lane 5: Mouse stomach tissue
Lane 6: Mouse liver tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 26, 24 kDa
Observed band size: 26 kDa
| Immunofluorescence staining of A549 cells with CSB-PA006009LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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