EGFR (Ab-1172) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | EC 2.7.10.1, epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian), Epidermal growth factor receptor precursor, ERBB1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P00533 | | Иммуноген | Synthesized non-phosphopeptide derived from human EGFR around the phosphorylation site of tyrosine 1172 (P-D-Y(p)-Q-Q). | | Источник | Rabbit | | Видовая специфичность | Human, Mouse | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Isoform 2 may act as an antagonist of EGF action.<br/><br/>Ullrich A., Nature 309:418-425(1984).<br/>Ilekis J.V., Mol. Reprod. Dev. 41:149-156(1995).<br/>Reiter J.L., Nucleic Acids Res. 24:4050-4056(1996)<br/> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Cancer,Signal transduction | | Ссылка на страницу на сайте производителя | ссылка | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using EGFR (Ab-1172) antibody.
 | Western blot analysis of extracts from HT-29 cells, using EGFR (Ab-1172) antibody.
 | Western blot analysis of extracts from A549 cells (Lane 2) and HepG2 cells (Lane 3), using EGFR (Ab-1172) antiobdy. The lane on the left is treated with synthesized peptide.
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Наименование: EGFR (Ab-1172) Antibody.