EGLN1 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Egl nine homolog 1 (EC 1.14.11.29) (Hypoxia-inducible factor prolyl hydroxylase 2) (HIF-PH2) (HIF-prolyl hydroxylase 2) (HPH-2) (Prolyl hydroxylase domain-containing protein 2) (PHD2) (SM-20), EGLN1, C1orf12 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | Q9GZT9 | | Иммуноген | Recombinant Human Egl nine homolog 1 protein (301-426AA) | | Источник | Rabbit | | Видовая специфичность | Human, Rat | | Применение | ELISA, WB, IHC, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200 | | Примечание | Cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1A. Also hydroxylates HIF2A. Has a preference for the CODD site for both HIF1A and HIF1B. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. EGLN1 is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Target proteins are preferencially recognized via a LXXLAP motif. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen Affinity Purified | | Области исследований | Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism | | Ссылка на страницу на сайте производителя | ссылка | Western blot
All lanes: Egl nine homolog 1 antibody at 12µg/ml + Rat brain tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 47, 44, 37 kDa
Observed band size: 47 kDa
 | Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA863932ESR2HU at dilution of 1:100
 | | | |
Информация для заказа |
Наименование: EGLN1 Antibody.