| Объем | 100 мкг |
| Синонимы | ER membrane protein complex subunit 8 (Neighbor of COX4) (Protein FAM158B), EMC8, C16orf2 C16orf4 COX4AL COX4NB FAM158B NOC4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | O43402 |
| Иммуноген | Recombinant Human ER membrane protein complex subunit 8 protein (1-210AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | cytoplasm, ER membrane protein complex, membrane, mitochondrion, nucleus |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: EMC8 antibody at 0.8µg/ml
Lane 1: A549 whole cell lysate
Lane 2: Human placenta tissue
Lane 3: Hela whole cell lysate
Lane 4: HT29 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 24, 14 kDa
Observed band size: 24 kDa
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Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA005835LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA005835LA01HU at dilution of 1:100
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Immunofluorescence staining of MCF-7 cells with CSB-PA005835LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Наименование: EMC8 Antibody.