| Объем | 50 мкл |
| Синонимы | F-box only protein 4, FBXO4, FBX4 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q9UKT5 |
| Иммуноген | Recombinant Human F-box only protein 4 protein (138-387AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IP:1:200-1:2000 |
| Примечание | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes ubiquitination of CCND1 and its subsequent proteasomal degradation. Recognizes TERF1 and promotes its ubiquitination together with UBE2D1. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | Antigen Affinity Purified |
| Абревеатура | F-box only protein 4 |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: FBXO4 antibody at 3.1µg/ml
Lane 1: 293T whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: HL60 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 45, 36 kDa
Observed band size: 45 kDa
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Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA891538ESR1HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA891538ESR1HU at dilution of 1:100
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Immunoprecipitating FBXO4 in HepG2 whole cell lysate
Lane 1: Rabbit monoclonal IgG (1µg) instead of CSB-PA891538ESR1HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)
Lane 2: CSB-PA891538ESR1HU (4µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (20µg)
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Наименование: FBXO4 Antibody.