| Объем | 100 мкг |
| Синонимы | F-box/WD repeat-containing protein 5 (F-box and WD-40 domain-containing protein 5), FBXW5, FBW5 |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | Q969U6 |
| Иммуноген | Recombinant Human F-box/WD repeat-containing protein 5 protein (265-494AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Substrate recognition component of both SCF (SKP1-CUL1-F-box protein) and DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes. Substrate recognition component of the SCF(FBXW5) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of SASS6 during S phase, leading to prevent centriole reduplication. The SCF(FBXW5) complex also mediates ubiquitination and degradation of actin-regulator EPS8 during G2 phase, leading to the transient degradation of EPS8 and subsequent cell shape changes required to allow mitotic progression. Substrate-specific adapter of the DCX(FBXW5) E3 ubiquitin-protein ligase complex which mediates the polyubiquitination and subsequent degradation of TSC2. May also act as a negative regulator of MAP3K7/TAK1 signaling in the interleukin-1B (IL1B) signaling pathway. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Абревеатура | F-box/WD repeat-containing protein 5 |
| Области исследований | Cell biology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: FBXW5 antibody at 2µg/ml + PC-3 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 64, 43 kDa
Observed band size: 64 kDa
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Immunohistochemistry of paraffin-embedded human appendix tissue using CSB-PA846577LA01HU at dilution of 1:100
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Immunofluorescent analysis of Hela cells using CSB-PA846577LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: FBXW5 Antibody.