| Объем | 100 мкг |
| Синонимы | IgG receptor FcRn large subunit p51 (FcRn) (IgG Fc fragment receptor transporter alpha chain) (Neonatal Fc receptor), FCGRT, FCRN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P55899 |
| Иммуноген | Recombinant Human IgG receptor FcRn large subunit p51 protein (24-297AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse |
| Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:1000, IF:1:50-1:200 |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Immunology |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: FCGRT antibody at 2µg/ml
Lane 1: HepG2 whole cell lysate
Lane 2: Mouse liver tissue
Lane 3: A549 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
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Western blot
All lanes: FCGRT antibody at 5.3 µg/ml
Lane 1: Mouse liver tissue
Lane 2: Mouse kidney tissue
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
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Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA008545EA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA008545EA01HU at dilution of 1:100
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Immunofluorescent analysis of HepG2 cells using CSB-PA008545EA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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IHC image of CSB-PA008545EA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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IHC image of CSB-PA008545EA01HU diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Наименование: FCGRT Antibody.