FEN1 AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Flap endonuclease 1, EC 3.1.-.-, Flap structure-specific endonuclease 1, FEN-1, Maturation factor 1 | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P39748 | | Иммуноген | Synthesized peptide derived from internal of human FEN1. | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF,WB:1:500-1:3000, IHC:1:50-1:100, IF:1:100-1:500 | | Примечание | Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.<br/><br/>Murray J.M., Mol. Cell. Biol. 14:4878-4888(1994).<br/>Hiraoka L.R., Genomics 25:220-225(1995).<br/>Taylor T.D., Nature 440:497-500(2006). | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | Western blot analysis of extracts from Jurkat cells, 293 cells and HUVEC cells, using FEN1 antibody.
 | Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using FEN1 antibody.
 | Immunofluorescence analysis of HeLa cells, using FEN1 antibody.
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Наименование: FEN1 Antibody.