Объем | 50 мкг |
Синонимы | Filamin A-interacting protein 1-like (130 kDa GPBP-interacting protein) (90 kDa GPBP-interacting protein) (Protein down-regulated in ovarian cancer 1) (DOC-1), FILIP1L, COL4A3BPIP DOC1 GIP90 |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q4L180 |
Иммуноген | Recombinant Human Filamin A-interacting protein 1-like protein (1-200AA) |
Источник | Rabbit |
Видовая специфичность | Human, Mouse, Rat |
Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:500, IF:1:50-1:500 |
Примечание | Acts as a regulator of the antiangiogenic activity on endothelial cells. When overexpressed in endothelial cells, leads to inhibition of cell proliferation and migration and an increase in apoptosis. Inhibits melanoma growth When expressed in tumor-associated vasculature. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Области исследований | Cancer, Cardiovascular |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: Rat stomach tissue, Mouse kidney tissue
All lanes: FILIP1L antibody at 2.5µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 131, 103, 30, 100, 80 kDa
Observed band size: 131 kDa
|
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA679703LA01HU at dilution of 1:100
|
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA679703LA01HU at dilution of 1:100
|
Immunofluorescence staining of HepG2 cells with CSB-PA679703LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
|
| |