| Объем | 50 мкг |
| Клон ID | 4G5C8 |
| Синонимы | 3X Flag, DDK tag, DYKDDDDK, Flag, Flag Tag |
| Тип антител | Tag/Control Antibodies |
| Иммуноген | DYKDDDDK(FLAG) synthetic peptide conjugate to KLH |
| Источник | Mouse |
| Применение | ELISA,WB,IF,IP,Recommended dilution:WB:1:500-1:5000,IF:1:50-1:200,IP:1:200-1:2000 |
| Изотип | IgG2b |
| Коньюгат | Non-conjugated |
| Метод очистки | >95%,Protein G purified |
| Буффер | Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Примечание | FLAG-tag, or FLAG octapeptide, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A FLAG-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a FLAG-tag to this protein allows one to follow the protein with an antibody against the FLAG sequence. Examples are cellular localization studies by immunofluorescence or detection by SDS PAGE protein electrophoresis. |
| Ссылка на страницу на сайте производителя | ссылка |
Western Immunoblot analysis of randomly picked 7 differend DDK-tagged overexpressio lysates with CSB-MA000021M0m at 1:2000
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IF:Hela cells transfected with DDK-tagged CD146 ORF cDNA clone were immunostained with M0002 antibody,and then colored in greed with FITC conjugated Secondary antibody(1:2000).The nuclei(blue) were counterstained with DAPI.
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IP-WB:Result of anti DDK-tag Monoclonal antibody
Lane 1:DDK-tagged fusion protein
Transfected HEK-293 cells lysate
Lane 2:Elution
Lane 3:Precipitateing DDK-tagged fusion protein
Secondary
Goat polyclonal to Mouse IgG at 1/5000 dilution
Predicted band size : 40kd
Observed band size : 40kd
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Наименование: Flag Tag Monoclonal Antibody.