| Объем | 50 мкг |
| Синонимы | Galactose-1-phosphate uridylyltransferase (Gal-1-P uridylyltransferase) (EC 2.7.7.12) (UDP-glucose--hexose-1-phosphate uridylyltransferase), GALT |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P07902 |
| Иммуноген | Recombinant Human Galactose-1-phosphate uridylyltransferase protein (1-379AA) |
| Источник | Rabbit |
| Видовая специфичность | Human, Mouse, Rat |
| Применение | ELISA, WB, IHC, IF, IP, Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Примечание | Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Cancer, Metabolism, Signal transduction |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: GALT antibody at 2µg/ml + Mouse small intestine tissue
Secondary
Goat polyclonal to rabbit IgG at 1/15000 dilution
Predicted band size: 44, 32 kDa
Observed band size: 44 kDa
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Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA009226LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA009226LA01HU at dilution of 1:100
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Western Blot
Positive WB detected in: Rat liver tissue, Mouse kidney tissue
All lanes: GALT antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 44, 32 kDa
Observed band size: 44 kDa
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Immunofluorescent analysis of Hela cells using CSB-PA009226LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Immunoprecipitating GALT in HepG2 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA009226LA01HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA009226LA01HU (8µg) + HepG2 whole cell lysate (500µg)
Lane 3: HepG2 whole cell lysate (10µg)
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Наименование: GALT Antibody.