GST AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Glutathione S Transferase, Glutathione S transferase Mu 1, Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, GST, GST class mu 1, GST1, GSTM1 1, GSTM1a 1a, GSTM1b 1b, GTH4, GTM1, H B, HB subunit 4, MGC26563, MU 1, MU, SJ26 antigen, SjGST. | | Клональность | Polyclonal Antibody | | Иммуноген | Purified recombinant Escherichia coli BL21(DE3) GST protein fragments expressed in E.coli. | | Источник | Rabbit | | Применение | ELISA, WB,WB:1:500-1:1000 | | Примечание | <p>The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial,and microsomal (now designated as MAPEG) proteins.GSTs are present in eukaryotes and in prokaryotes,where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates.Members of the GST superfamily are extremely diverse in amino acid sequence,and a large fraction of the sequences deposited in public databases are of unknown function.The Enzyme Function Initiative (EFI) is using GSTs as a model superfamily to identify new GST functions.GSTs can constitute up to 10% of cytosolic protein in some mammalian organs.GSTs catalyse the conjugation of reduced glutathione &mdash, via a sulfhydryl group &mdash, to electrophilic centers on a wide variety of substrates in order to make the compounds more soluble.This activity detoxifies endogenous compounds such as peroxidised lipids, as well as breakdown of xenobiotics.GSTs may also bind toxins and function as transport proteins,which gave rise to the early term for GSTs of &ldquo,ligandin&rdquo,.The mammalian GST super-family consists of cytosolic dimeric isoenzymes of 45&ndash,55 kDa size that have been assigned to at least six classes:Alpha,Mu,Pi,Theta,Zeta and Omega.Most mammalian isoenzymes have affinity for the substrate 1-chloro-2,4-dinitrobenzene (CDNB),and spectrophotometric assays utilising this substrate are commonly used to report GST activity.However,some endogenous compounds,e.g., bilirubin,can inhibit the activity of GSTs.In mammals,GST isoforms have cell specific distributions (e.g., alpha GST in hepatocytes and pi GST in the biliary tract of the human liver).</p> | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Purified Rabbit polyclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol. | | Форма | liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Affinity-chromatography | | Области исследований | Others | | Ссылка на страницу на сайте производителя | ссылка | Western blot detection of GST antibody in CHO-K1 transfected with GST cell lysate and GST recombinant protein fragmentsUrified recombinant human GST-Lin28B & GST-Lin28A protein fragments expressed in E.coli,using GST antibody (1:10000 diluted).
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Наименование: GST Antibody.