Объем | 100 мкг |
Синонимы | Hepatocyte cell adhesion molecule (Protein hepaCAM), HEPACAM |
Клональность | Polyclonal Antibody |
Организм | Human |
uniprot | Q14CZ8 |
Иммуноген | Recombinant Human Hepatocyte cell adhesion molecule protein (265-415AA) |
Источник | Rabbit |
Видовая специфичность | Human |
Применение | ELISA, WB, IF, Recommended dilution: WB:1:500-1:5000, IF:1:200-1:500 |
Примечание | Involved in regulating cell motility and cell-matrix interactions. May inhibit cell growth through suppression of cell proliferation. |
Клональность1 | Polyclonal |
Изотип | IgG |
Коньюгат | Non-conjugated |
Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Форма | Liquid |
Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
Метод очистки | >95%, Protein G purified |
Абревеатура | Hepatocyte cell adhesion molecule |
Области исследований | Cancer, Signal transduction |
Ссылка на страницу на сайте производителя | ссылка |
Western Blot
Positive WB detected in: MCF-7 whole cell lysate, HepG2 whole cell lysate
All lanes: HEPACAM antibody at 3.2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47, 41 kDa
Observed band size: 47 kDa
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Immunofluorescence staining of MCF-7 cells with CSB-PA010287LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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