| Объем | 50 мкг |
| Синонимы | Histone H2A type 1 (H2A.1) (Histone H2A/ptl), HIST1H2AG, HIST1H2AI, HIST1H2AK, HIST1H2AL, HIST1H2AM, H2AFP, H2AFC, H2AFD, H2AFI, H2AFN |
| Клональность | Polyclonal Antibody |
| Организм | Human |
| uniprot | P0C0S8 |
| Иммуноген | Recombinant Human Histone H2A type 1 protein (2-130AA) |
| Источник | Rabbit |
| Видовая специфичность | Human |
| Применение | ELISA, WB, IHC, IF, ChIP, Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200 |
| Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Клональность1 | Polyclonal |
| Изотип | IgG |
| Коньюгат | Non-conjugated |
| Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Форма | Liquid |
| Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Метод очистки | >95%, Protein G purified |
| Области исследований | Others |
| Ссылка на страницу на сайте производителя | ссылка |
Western blot
All lanes: HIST1H2AG antibody at 4µg/ml + HL60 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
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Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010389LA01HU at dilution of 1:100
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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA010389LA01HU at dilution of 1:100
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Chromatin Immunoprecipitation Hela (1.2*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-HIST1H2AG or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP010389HU) against the PGK1 promoter.
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Immunofluorescent analysis of Hela cells using CSB-PA010389LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Наименование: HIST1H2AG Antibody.