HIST1H2BC (Ab-12) AntibodyСпецификация| Объем | 100 мкл | | Синонимы | Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC, HIST1H2BE, HIST1H2BF, HIST1H2BG, HIST1H2BI, H2BFL, H2BFH, H2BFG, H2BFA, H2BFK | | Клональность | Polyclonal Antibody | | Организм | Human | | uniprot | P62807 | | Иммуноген | Peptide sequence around site of acetyl-Lys (12) derived from Human Histone H2B | | Источник | Rabbit | | Видовая специфичность | Human, Mouse, Rat | | Применение | ELISA, WB, IHC, IF, Recommended dilution: WB:1:200-1:2000, IHC:1:10-1:100, IF:1:50-1:200 | | Примечание | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. | | Клональность1 | Polyclonal | | Изотип | IgG | | Коньюгат | Non-conjugated | | Буффер | Preservative: 0.03% Proclin 300<br />Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 | | Форма | Liquid | | Хранение | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | | Метод очистки | Antigen Affinity Purified | | Абревеатура | Histone H2B type 1-C/E/F/G/I | | Области исследований | Epigenetics and Nuclear Signaling | | Ссылка на страницу на сайте производителя | ссылка | Western Blot
Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, Rat liver tissue, Rat kidney tissue, Mouse brain tissue, Mouse spleen tissue
All lanes: HIST1H2BC antibody at 0.47µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
 | IHC image of CSB-PA010403OA12nacHU diluted at 1:20 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
 | Immunofluorescence staining of Hela cells with CSB-PA010403OA12nacHU at 1:3, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Информация для заказа| Область использования: | Производство: | Cusabio | | Метод: | Антитела | | Объем: | 100 мкл | | Кат. номер: | CSB-PA010403OA12nacHU | | Цена (с НДС 20%): | по запросу | В корзину  |  Наименование: HIST1H2BC (Ab-12) Antibody.
Примечание: дополнительная информация (на английском языке). |
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